Open AccessМониторинг изменения содержания кислорода в тканях по кинетике замедленной флуоресценции экзогенных красителей
Author(s) -
С.Н. Летута,
С.Н. Пашкевич,
Э.К. Алиджанов,
Ю.Д. Лантух,
Д.А. Раздобреев,
А.А. Чакак,
А.Т. Ишемгулов
Publication year - 2019
Publication title -
žurnal tehničeskoj fiziki
Language(s) - English
Resource type - Journals
eISSN - 1726-748X
pISSN - 0044-4642
DOI - 10.21883/os.2019.12.48706.377-18
Subject(s) - singlet oxygen , xanthene , photodynamic therapy , photochemistry , quenching (fluorescence) , oxygen , fluorescence , chemistry , photosensitizer , luminescence , triplet state , oxygen tension , biophysics , materials science , optoelectronics , optics , biology , organic chemistry , molecule , physics
The dynamics of oxygen consumption during photodynamic processes and its subsequent restoration in malignant tumors and healthy tissues of mice were studied in vitro by kinetics of long-term luminescence of xanthene dyes. It was shown that to estimate changes in tissue oxygen tension, specific type of delayed fluorescence can be used which caused by singlet-triplet annihilation of singlet oxygen and a sensitizer in triplet state. In tumors under pulse excitation of sensitizers, reversible quenching of delayed fluorescence was observed, which is associated with a decrease in the tissue oxygen tension during photodynamic processes. Method for visualizing the restoration of the initial oxygen level in tissues after photodynamic action is proposed.