Open AccessИсследование влияния материала микрофлюидного чипа на полимеразную цепную реакцию
Author(s) -
А.Н. Тупик,
Г.Е. Рудницкая,
T. A. Lukashenko,
А.А. Евстрапов
Publication year - 2020
Publication title -
žurnal tehničeskoj fiziki
Language(s) - English
Resource type - Journals
eISSN - 1726-748X
pISSN - 0044-4642
DOI - 10.21883/jtf.2020.09.49692.436-19
Subject(s) - polymerase chain reaction , adsorption , polymer , chemical engineering , chemistry , materials science , chromatography , organic chemistry , biochemistry , gene , engineering
The effect of a number of polymer materials of domestic and foreign manufacturers on the polymerase chain reaction has been verified. It was found that in the microchip from polymethylmethacrylate there was no adsorption of the components of the reaction mixture at room temperature, however, during the polymerase reaction in the microchip, the reaction efficiency decreases at low DNA concentrations.