Biosurfactant-Producing Bacteria Isolated from Oil Contaminated Soil and its Media Optimization for Enzyme Production

Oil bio-degradation mechanism by microorganisms is requested for an effective microbial remediation of soil contamination by oil spills. The current examination pointed the identification of a biosurfactant producing bacteria for biosurfactant production from oil contaminated sites from Tamil Nadu. The biosurfactant testing screening methods were used to screen the potent strains and sequencing studies were used for Pseudomonas species identification. The bacterial isolate BS17 subjected to be the potent enzyme (Protease, Lipase and Esterase) producer. Among the tested production media, the ground nut oil cake was identified to be the optimum media for protease (0.47069 Unit/ml), lipase (9 Unit/ml) and esterase activity (3.891 Unit/ml) for bacterial isolate BS17. The bacterial isolate BS17 showed greatest lipase (15 Unit/mL) protease (0.8067 Unit/mL) and esterase (4.756 Unit/mL) enzyme activity at pH 9.0. At 35 ℃ bacterial isolate BS17 showed greatest enzyme action in protease (1.2772 Unit/mL), lipase (17 Unit/mL) and esterase (5.2972 Unit/mL) enzyme activity. At 48hrs of incubation period bacterial isolate BS17 showed most extreme enzyme activity in protease (3.361 Unit/mL), lipase (28 Unit/mL) and esterase (8.918 Unit/mL). The sequence of BS17 was deposited in NCBI and Accession number was received [MT337593.1]. Statistical analysis with the minimum significant difference (LSD) test of ANOVA was carried out to determine the oil degradation efficiency. This paper demonstrated the isolated P. aeruginosa (BS17) crude oil biodegradation from oil contaminated land soil sample. Strain BS17 was proved as potent bio-surfactant producer using crude oil by utilizing carbon and energy source in oil degradation mechanism.