Open AccessTHE EFFECT OF GLUT4 EXPRESSION IN HIPPOCAMPAL NEURONS TO SPATIAL MEMORY OF DIABETES-INDUCED RATTUS NOVERGICUS
Author(s) -
Herpan Syafii Harahap,
Novrita Padauleng,
Rizka Vidya Lestari,
Nurhidayati Nurhidayati,
Anak Agung Ayu Niti Wedayani,
Putu Suwita Sari,
Hifdzil Haq Muliyana,
Ngakan Putu Proudy Laksmana,
Aliza Raudatin Sahly
Publication year - 2021
Publication title -
mnj (malang neurology journal)
Language(s) - English
Resource type - Journals
eISSN - 2442-5001
pISSN - 2407-6724
DOI - 10.21776/ub.mnj.2021.007.02.6
Subject(s) - glut4 , hippocampal formation , diabetes mellitus , glucose transporter , hippocampus , immunohistochemistry , medicine , endocrinology , streptozotocin , biology , insulin
Background: Identification of appropriate biomarker involved in the pathophysiology of diabetes-associated cognitive decline in animal model is necessary. Glucose transporter 4 (GLUT4) is the component of insulin-dependent neuronal signaling pathway needed for maintaining cognitive function. It is highly expressed in hippocampal neurons. Objective: To investigate the effect of GLUT4 expression in hippocampal neurons on the spatial memory of diabetes-induced Rattus novergicus. Methods: This experimental study used 24 male Rattus novergicus divided into control groups terminated on day-0 (C0), 14 (C1) and 28 (C2) and diabetic groups terminated on day-0 (D0), 14 (D1) and 28 (D2). Diabetes was induced using streptozotocin injections intraperitoneally. Spatial memory (travel time) of rats was assessed on day-14 and 28. Brain tissue sampling was performed for assessment of GLUT4 expression using immunohistochemical methods. Significant differences in means of GLUT4 expression and travel time between groups on day-0, 14 and 28 as well as correlation between means of GLUT4 expression and travel time in D1 and D2 were analyzed statistically. Results: The mean of GLUT4 expression in diabetic group was significantly higher compared to control on day-14, but not on day-0 and 28. The immunohistochemical examination confirmed this results. There were no significant differences in mean of travel time between control and diabetic groups on day-14 and 28. There were no significant correlation between means of GLUT4 expression and travel time in diabetic groups on day-14 and 28. Conclusion: The GLUT4 expression of hippocampal neurons did not significantly affect spatial memory of diabetes-induced Rattus novergicus.