Open AccessStatus and Prospects of Next Generation Sequencing Technologies in Crop Plants
Author(s) -
Tilak Raj Sharma,
Awadhesh Kumar,
Kanti Kiran,
Pankaj Kumar Singh,
Kirti Arora,
Priyanka Jain,
Ila M. Tiwari,
Himanshu Dubey,
Banita Kumari Saklani,
Mandeep Kumari,
Jaya Singh,
Rajdeep Jaswal,
Ritu Kapoor,
Deepak Pawar,
Shruti Sinha,
Deepak Singh Bisht,
Amolkumar U. Solanke,
Tapan Kumar Mondal
Publication year - 2018
Publication title -
current issues in molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 53
eISSN - 1467-3045
pISSN - 1467-3037
DOI - 10.21775/cimb.027.001
Subject(s) - dna sequencing , sanger sequencing , metagenomics , biology , computational biology , genome , genomics , microbiology and biotechnology , genetics , dna , gene
The history of DNA sequencing dates back to 1970s. During this period the two first generation nucleotide sequencing techniques were developed. Subsequently the Sanger's dideoxy method of sequencing gained popularity over Maxam and Gilbert's chemical method of sequencing. However, in the last decade, we have observed revolutionary changes in DNA sequencing technologies leading to the emergence of next-generation sequencing (NGS) techniques. NGS technologies have enhanced the throughput and speed of sequencing combined with bringing down the overall cost of the process over a time. The major applications of NGS technologies being genome sequencing and resequencing, transcriptomics, metagenomics in relation to plant-microbe interactions, exon and genome capturing, development of molecular markers and evolutionary studies. In this review, we present a broader picture of evolution of NGS tools, its various applications in crop plants, and future prospects of the technology for crop improvement.