Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
Author(s) -
Tim Orthwein,
Luciano F. Huergo,
Karl Forchhammer,
Khaled A. Selim
Publication year - 2021
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.4152
Subject(s) - analyte , chemistry , analytical chemistry (journal) , dissociation constant , kinetics , wavelength , dissociation (chemistry) , biosensor , chromatography , materials science , optoelectronics , biochemistry , physics , receptor , quantum mechanics
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