Open AccessQuantification of Starch in Guard Cells of Arabidopsis thaliana
Author(s) -
Sabrina Flütsch,
Luca Distefano,
Diana Santelia
Publication year - 2018
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.2920
Subject(s) - guard cell , propidium iodide , fluorophore , arabidopsis thaliana , starch , arabidopsis , confocal microscopy , confocal laser scanning microscopy , chemistry , microbiology and biotechnology , biophysics , botany , biology , fluorescence , biochemistry , physics , optics , gene , apoptosis , programmed cell death , mutant
In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.