Open AccessMeasurement of ROS in Caenorhabditis elegans Using a Reduced Form of Fluorescein
Author(s) -
Shaarika Sarasija,
Kenneth R. Norman
Publication year - 2018
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.2800
Subject(s) - caenorhabditis elegans , reactive oxygen species , oxidative stress , mitochondrion , mitochondrial ros , microbiology and biotechnology , oxidative damage , pathogenesis , biology , oxidative phosphorylation , chemistry , biochemistry , immunology , gene
Oxidative stress is implicated in the pathogenesis of various neurodegenerative diseases, including Alzheimer's disease. Oxidative stress is a result of a disruption of the equilibrium between antioxidants and oxidants, in favor of oxidants. Since mitochondria are major sites of production and reduction of reactive oxygen species (ROS), measurement of ROS levels can help us determine if mitochondrial functional integrity has been compromised. In this protocol, we describe a method to measure the level of ROS in the nematode Caenorhabditis elegans, using chloromethyl-2,7'-dichlorodihydrofluorescein diacetate (CM-H 2 DCFDA).