Open AccessBehavioral Assays to Study Oxygen and Carbon Dioxide Sensing in Caenorhabditis elegans
Author(s) -
Teresa Rojo Romanos,
Ley Moy Ng,
Manuel Zimmer,
Roger Pocock
Publication year - 2018
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.2679
Subject(s) - caenorhabditis elegans , population , carbon dioxide , biology , neuroscience , ecology , gene , genetics , medicine , environmental health
Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses to changes in environmental oxygen (O 2 ) and carbon dioxide (CO 2 ) concentrations. These behavioral assays are high-throughput and enable examination of genetic, neuronal and circuit function.