Determining Ribosome Translational Status by Ribo-ELISA | Zendy

Amandine Bastide | Zendy; Jonathan W. Yewdell | Zendy; Alexandre David | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Determining Ribosome Translational Status by Ribo-ELISA

Author(s) -

Amandine Bastide,

Jonathan W. Yewdell,

Alexandre David

Publication year - 2017

Publication title -

bio-protocol

Language(s) - English

Resource type - Journals

ISSN - 2331-8325

DOI - 10.21769/bioprotoc.2670

Subject(s) - ribosome , ribosomal rna , translation (biology) , macromolecule , eukaryotic ribosome , ribosomal protein , chemistry , computational biology , biology , biochemistry , rna , messenger rna , gene

The Ribo-ELISA was originally developed to elucidate the basis for the ribopuromycylation method (RPM)-based detection of ribosome bound nascent chains. The Ribo-ELISA enables characterization of the translational status of ribosomes, and can be applied to the discovery of super-ribosomal complexes with novel ribosome associated macromolecules that are isolated by physical fractionation in sucrose gradients or other methods.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research