Open AccessDetermining Ribosome Translational Status by Ribo-ELISA
Author(s) -
Amandine Bastide,
Jonathan W. Yewdell,
Alexandre David
Publication year - 2018
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.2670
Subject(s) - ribosome , ribosomal rna , translation (biology) , macromolecule , eukaryotic ribosome , ribosomal protein , chemistry , computational biology , biology , biochemistry , rna , messenger rna , gene
The Ribo-ELISA was originally developed to elucidate the basis for the ribopuromycylation method (RPM)-based detection of ribosome bound nascent chains. The Ribo-ELISA enables characterization of the translational status of ribosomes, and can be applied to the discovery of super-ribosomal complexes with novel ribosome associated macromolecules that are isolated by physical fractionation in sucrose gradients or other methods.