Open AccessStereotaxic Adeno-associated Virus Injection and Cannula Implantation in the Dorsal Raphe Nucleus of Mice
Author(s) -
Patrícia A. Correia,
Sara Matias,
Zachary F. Mainen
Publication year - 2017
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.2549
Subject(s) - optogenetics , dorsal raphe nucleus , neuroscience , raphe nuclei , tail vein , cannula , raphe , biology , suprachiasmatic nucleus , serotonergic , serotonin , medicine , central nervous system , surgery , in vivo , biochemistry , receptor , microbiology and biotechnology
Optogenetic methods are now widespread in neuroscience research. Here we present a detailed surgical procedure to inject adeno-associated viruses and implant optic fiber cannulas in the dorsal raphe nucleus (DRN) of living mice. Combined with transgenic mouse lines, this protocol allows specific targeting of serotonin-producing neurons in the brain. It includes fixing a mouse in a stereotaxic frame, performing a craniotomy, virus injection and fiber implantation. Animals can be later used in behavioral experiments, combined with optogenetic manipulations ( Dugué et al ., 2014 ; Correia et al ., 2017 ) or monitoring of neuronal activity ( Matias et al ., 2017 ). The described procedure is a fundamental step in both optogenetic and fiber photometry experiments of deep brain areas. It is optimized for serotonin neurons in the DRN, but it can be applied to any other cell type and brain region. When using transgenic mouse lines that express functionally relevant levels of optogenetic tools or reporter lines, the virus injection step can be skipped and the protocol is reduced to the cannula implantation procedure.