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Molecular biological methods in environmental engineering
Author(s) -
Li Chunying,
Pulin Zhou,
Wei Li,
Wei Dong,
Jia Ouyang,
Chang CheinChi
Publication year - 2018
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143018x15289915807461
Subject(s) - biochemical engineering , environmental dna , bioremediation , computational biology , genomics , environmental monitoring , environmental pollution , biology , environmental science , engineering , ecology , genome , biodiversity , environmental protection , genetics , contamination , gene
Environmental bioremediation technologies are undoubtedly more economical and environmental friendly, and present a good application prospect. Microorganisms can be used to monitor environmental changes for their quickly respond to environmental changes. Microbiological molecular biology methods have attracted more and more attention from human beings, and their applications in the environment is also expanding. Genomics, transcriptomics, proteomics, and metabolomics are four major molecular biology techniques. This review summarizes application of molecular biological methods in environmental engineering in 2017. The summary includes methods for DNA, RNA, and protein extraction, the application of four major technologies in the environmental field, and commonly used methods for in situ monitoring in the environment, including fluorescence in situ hybridization, real‐time quantitative PCR, and amplicons sequencing technology.