Premium
Pathogen Characterization of Fresh and Stored Mesophilic Anaerobically Digested Biosolids
Author(s) -
Flemming Cecily A.,
Simhon Albert,
Odumeru Joseph A.
Publication year - 2017
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143017x14839994522704
Subject(s) - biosolids , cryptosporidium parvum , microbiology and biotechnology , salmonella , cryptosporidium , listeria monocytogenes , biology , giardia lamblia , indicator bacteria , clostridium perfringens , giardia , food science , yersinia enterocolitica , bacteria , fecal coliform , feces , environmental science , environmental engineering , water quality , ecology , genetics
Culturable bacterial pathogens ( Campylobacter , Salmonella , Listeria , Yersinia ) and indicators ( E. coli , enterococci, Clostridium perfringens ) were quantified at six water resource recovery facilities that land apply anaerobically digested biosolids in Ontario, Canada. Cryptosporidium parvum and Giardia lamblia were also quantified by polymerase chain reaction (PCR). Salmonella and Listeria were frequently detected in sludge and liquid biosolids (70–100% of samples) but less often in fresh dewatered cake biosolids (50–60%); with low levels in fresh cake (<100 cells/g dw). Yersinia were in 20 to 30% of samples, typically at very low levels (<10 cell/g dw). Giardia and Cryptosporidium were detected in 80 and 20% of cake biosolids at geometric means of 270 cysts/g dw and 70 oocysts/g dw, respectively. E. coli reduction was typically >2‐log 10 while pathogen reduction was variable. “Sudden increase” of pathogens was not observed, however, Salmonella and E. coli showed regrowth (at 1 to 3 orders of magnitude) after 2‐ to 3‐day storage at 30 °C.