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Molecular Biological Methods in Environmental Engineering
Author(s) -
Zhang Guocai,
Wei Li,
Chang CheinChi,
Zhang Yuhua,
Wei Dong
Publication year - 2016
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143016x14696400494371
Subject(s) - pyrosequencing , computational biology , biology , identification (biology) , dna sequencing , microbial population biology , function (biology) , biochemical engineering , microbial genetics , bacteria , microbiology and biotechnology , gene , ecology , genetics , engineering
Bacteria, acting as catalysts, perform the function of degrading pollutants. Molecular biological techniques play an important role in research on the community analysis, the composition and the functions of complex microbial communities. The development of secondary high‐throughput pyrosequencing techiniques enhances the understanding of the composition of the microbial community. The literatures of 2015 indicated that 16S rDNA gene as genetic tag is still the important method for bacteria identification and classification. 454 high throughput sequencing and Illumina MiSeq sequencing have been the primary and widely recognized methods to analyze the microbial. This review will provide environmental engineers and microbiologists an overview of important advancements in molecular techniques and highlight the application of these methods in diverse environments.