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Control of Attachment of Pseudomonas aeruginosa and Burkholderia cepacia to Surfaces by Shear Force
Author(s) -
Hui Yew Woh,
Narayanan Kumaran,
Dykes Gary A.
Publication year - 2016
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143016x14504669767292
Subject(s) - burkholderia , pseudomonas aeruginosa , shearing (physics) , polystyrene , microbiology and biotechnology , pseudomonadaceae , chemistry , pseudomonas , pseudomonadales , shear force , bacteria , materials science , biology , composite material , polymer , organic chemistry , genetics
The effect of physical shearing on the attachment of six Pseudomonas aeruginosa strains and six Burkholderia cepacia strains to glass, stainless steel, polystyrene and Teflon® was determined. A significant ( p < 0.05) decrease in hydrophobicity was apparent for all P. aeruginosa strains (17–36%) and B. cepacia, MS 5 (20%) after shearing. A significant ( p < 0.05) decrease in attachment of some P. aeruginosa (0.2–0.5 log CFU/cm 2 ) and B. cepacia (0.2–0.4 log CFU/cm 2 ) strains to some surface types was apparent after shearing. Significant ( p < 0.05) correlation was observed for both numbers of flagellated cells and hydrophobicity against attachment to glass, stainless steel and polystyrene for P. aeruginosa while only hydrophobicity showed significant correlation against the same surfaces for B. cepacia . Scanning electron microscopy and protein analysis showed that shearing removed surface proteins from the cells and may have led to the observed changes in hydrophobicity and attachment to abiotic surfaces.