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The Spatial Distribution of Nitrogen Removal Functional Genes in Multimedia Constructed Wetlands for Wastewater Treatment
Author(s) -
Ji Guodong,
He Chunguang,
Tan Yufei,
Yang Zhonghua
Publication year - 2015
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143014x13896437493463
Subject(s) - wastewater , wetland , constructed wetland , nitrogen , sewage treatment , environmental science , environmental engineering , spatial distribution , multimedia , computer science , waste management , ecology , engineering , geography , chemistry , biology , remote sensing , organic chemistry
The real‐time polymerase chain reaction was used to quantitatively evaluate distribution patterns and nitrogen removal pathways of the amoA , nxrA , narG , napA , nirK , qnorB , nosZ , nas , and nifH genes and 16S rRNA in anaerobic ammonia oxidation bacteria in four multimedia constructed wetlands for rural wastewater treatment. The results indicated that the abundance of functional genes for nitrogen removal in the rhizosphere layer (0 to 30 cm), water distribution layer (30 to 50 cm), multime filler layer (50 to 130 cm), and catchment layer (130 to170 cm) of the constructed wetlands were closely related. The rhizosphere layer was conducive to the absolute enrichment of dominant genes. The other three layers were favorable to the relative enrichment of rare genes.