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Detection and Occurrence of Indicator Organisms and Pathogens
Author(s) -
Kronlein Maggie R.,
Stedtfeld Robert D.,
Sorensen Jackson,
Bhaduri Prianca,
Stedtfeld Tiffany,
Eanes Samantha,
Harichandran Vikram,
Haynes Kathleen,
Stevens Michael,
Hashsham Syed A.
Publication year - 2013
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143013x13698672321706
Subject(s) - loop mediated isothermal amplification , feces , polymerase chain reaction , biology , microbiology and biotechnology , indicator bacteria , detection limit , immunoassay , fecal coliform , chromatography , dna , chemistry , ecology , genetics , water quality , gene , antibody
This review summarizes the literature published in 2012 pertaining to the occurrence and detection of fecal indicator bacteria (FIB) and pathogens. It is organized into the following sections: i) methods for the detection and quantification of waterborne fecal indicators and pathogens including polymerase chain reaction (PCR), isothermal amplification, label‐free methods, microarrays, immunoassay‐based methods, microfluidic‐based methods, and advances in sample processing and concentration at the limit of detection (LOD), ii) microbial source tracking (MST) using both genotypic and phenotypic‐based methods, iii) environmental occurrence, persistence, and transport of fecal indicators and pathogens.