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Molecular Characterization of Denitrifying Bacteria Isolated from the Anoxic Reactor of a Modified DEPHANOX Plant Performing Enhanced Biological Phosphorus Removal
Author(s) -
Zafiriadis Ilias,
Ntougias Spyridon,
Mirelis Paraskevi,
Kapagiannidis Anastasios G.,
Aivasidis Alexander
Publication year - 2012
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143012x13280358613543
Subject(s) - denitrifying bacteria , enhanced biological phosphorus removal , denitrification , microbiology and biotechnology , activated sludge , population , anoxic waters , bacteria , biology , chemistry , environmental chemistry , sewage treatment , environmental engineering , nitrogen , genetics , demography , organic chemistry , sociology , engineering
Enhanced Biological Phosphorus Removal (EBPR) under anoxic conditions was achieved using a Biological Nutrient Removal (BNR) system based on a modification of the DEPHANOX configuration. Double‐probe Fluorescence in Situ Hybridization (FISH) revealed that Polyphosphate Accumulating Organisms (PAOs) comprised 12.3 ± 3.2% of the total bacterial population in the modified DEPHANOX plant. The growing bacterial population on blood agar and Casitone Glycerol Yeast Autolysate agar (CGYA) medium was 16.7 ± 0.9 × 10 5 and 3.0 ± 0.6 × 10 5 colony forming units (cfu) mL −1 activated sludge, respectively. A total of 121 bacterial isolates were characterized according to their denitrification ability, with 26 bacterial strains being capable of reducing nitrate to gas. All denitrifying isolates were placed within the α‐, β‐, and γ‐subdivisions of Proteobacteria and the family Flavobacteriaceae. Furthermore, a novel denitrifying bacterium within the genus Pseudomonas was identified. This is the first report on the isolation and molecular characterization of denitrifying bacteria from EBPR sludge using a DEPHANOX‐type plant.