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Molecular Methods in Biological Systems
Author(s) -
Gu April Z.,
Nerenberg Robert,
Sturm Belinda M.,
Chul Park,
Goel Ramesh
Publication year - 2010
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143010x12756668800735
Subject(s) - terminal restriction fragment length polymorphism , computational biology , restriction fragment length polymorphism , temperature gradient gel electrophoresis , biology , data science , computer science , genetics , polymerase chain reaction , gene , 16s ribosomal rna
In 2009, molecular methods continue to be widely applied in different areas of environmental engineering research. Fingerprinting techniques such as denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T‐RFLP) and automated ribosomal intergenic spacer analysis (ARISA) continue to be popular tools to track differences in microbial community composition over time and space. Fluorescent in situ hybridization (FISH) of whole cells was also commonly employed to observe microbes of interest in ecological systems. Real time quantitative PCR methods appear to have become much more mainstream and were used to conduct comparative analyses of communities in different systems and over time. Several data analysis methods and software was proposed to analyze large and complex genomic data. In this review we chose to focus on new or significantly revised methods, novel applications of existing methods, and a few high quality reviews published in 2009, since the body of literature using molecular methods in environmental engineering has become very large.