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Factorial Experimental Designs for Enhancement of Concurrent Poly(Hydroxyalkanoate) Production and Brewery Wastewater Treatment
Author(s) -
Liu HsinYing,
VanderGheynst Jean S.,
Darby Jeannie L.,
Thompson Donald E.,
Green Peter G.,
Loge Frank J.
Publication year - 2011
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143010x12681059116932
Subject(s) - anoxic waters , hydraulic retention time , wastewater , factorial experiment , biomass (ecology) , pulp and paper industry , nitrogen , chemistry , carbon fibers , carbon source , sewage treatment , environmental science , environmental engineering , chromatography , environmental chemistry , mathematics , biology , biochemistry , ecology , organic chemistry , statistics , algorithm , composite number , engineering
The influence of four main process parameters—solids retention time (SRT), hydraulic retention time (HRT), anoxic‐oxic cycling, and carbon‐to‐nitrogen ratio (C/N ratio)—on poly(hydroxyalkanoate) (PHA) production, while treating brewery wastewater, was studied. Two sets of two‐level, three‐factor experimental designs were implemented to (1) determine the effects and interactions among process parameters, (2) assess their significance to PHA production, and (3) approximate optimal operational conditions. The HRT and SRT were found to be the crucial operational parameters affecting PHA production. The highest PHA content of 55% (on a cell‐weight basis) was produced at a 4‐day HRT and 4‐day SRT, whereas a maximum PHA concentration of 907 mg/L was obtained at a 2‐day HRT and 12‐day SRT. The effect of anoxic conditions on PHA production was insignificant. The C/N ratio played a more important role in the PHA concentration in the system than in the PHA content in the biomass.