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Microbial Enumeration of Different Functional Groups and Bacterial Behavior in Acid Basic Conditions of a Biotoxic Landfill Leachate of Bahía Blanca, Argentina
Author(s) -
Yunes Fabiana,
Baldini Mónica D.,
Gómez Marisa A.
Publication year - 2009
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143008x370467
Subject(s) - leachate , bacteria , nitrate , chemistry , environmental chemistry , wastewater , ammonium , lactuca , environmental engineering , botany , environmental science , biology , organic chemistry , genetics
Leachate is liquid waste from refuse biological decomposition or rainwater percolation in a landfill. This research focused on leachate produced by a landfill in Bahía Blanca, Buenos Aires, Argentina. The research studied the main microbial populations involved in wastewater treatment, analyzed the behavior of bacteria isolated from leachate at different pH values, and appraised leachate biotoxicity. The number of bacteria varied by type, ranging from 1 × 10 4 to 1 × 10 5 CUF/mL aerobic heterotrophic bacteria (AHB); 1 × 10 3 to 1 × 10 5 CUF/mL anaerobic heterotrophic bacteria (ANHB); 1 × 10 5 to 1 × 10 6 CUF/mL sulfite‐reducing bacteria (SRB); 1 × 10 3 to 1 × 10 6 NMP/mL nitrate‐reducing bacteria (NRB); and 1 × 10 2 to 1 × 10 4 NMP/mL ammonium‐oxidizing bacteria (AOB). 
Several microbial strains developed at pH 5, 7, and 10. These pH values changed to 9 in the culture media after a 48‐hour incubation. Leachate was used to water lettuce seeds ( Lactuca sativa capitata ). Its toxicity was proved by full inhibition of plant development.

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