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Alternative Immunofluorescent Labeling of Cryptosporidium parvum in Water Samples Using Semiconductor Quantum Dots
Author(s) -
Lee Lai Yoke,
Hu Jiang Yong,
Ong Say Leong,
Ng How Yong,
Wong Shih Wei,
Feng Yaoyu,
Tan Xiaolan
Publication year - 2008
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143008x276651
Subject(s) - turbidity , effluent , cryptosporidium parvum , cryptosporidium , wastewater , tap water , quantum dot , chemistry , fluorescence , chromatography , environmental chemistry , microbiology and biotechnology , environmental engineering , materials science , environmental science , nanotechnology , biology , physics , optics , ecology , feces
The application of immunofluorescent labeling using quantum dots for detection of inactivated Cryptosporidium parvum oocysts in spiked water samples (reservoir water, treated wastewater effluent, permeate of a membrane bioreactor, and tap water) provided more consistent results compared with the organic fluorophores label. The varying degree of particles present in the different water samples (with turbidity ranging from 0.2 to 6.1 NTU) in nonconcentrated water samples had insignificant interference on the labeled counts (2‐sample t ‐tests, p > 0.236) using the quantum dot label, while the quantum dot label provided an advantage of approximately 50% lower interference in concentrated water samples compared with the organic fluorophores label.