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Concentration and Recovery of Coliphages from Water with Bituminous Coal
Author(s) -
Dafale Nishant,
Lakhe Shrikumar,
Yadav Krishnakant,
Purohit Hemant,
Chakrabarti Tapan
Publication year - 2008
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143007x220923
Subject(s) - coliphage , adsorption , elution , chemistry , fecal coliform , wastewater , environmental chemistry , water pollution , chromatography , microbiology and biotechnology , environmental engineering , environmental science , biology , water quality , bacteriophage , escherichia coli , ecology , biochemistry , organic chemistry , gene
Coliphages represent a process indicator for fecal pollution. The coal bed concentration method prepared for enterovirus was refined for a concentration of coliphages. A bed made from 1.5 g of 120‐mesh coal powder was used for concentrating coliphage from 200 mL of a water sample with or without the addition of aluminum chloride at different pH values. The isolated E. coli strain EC‐R8 was found to be more susceptible to the desired coliphage and showed significant coliphage–coliform response, with clear plaque used for further studies. The complete coliphage adsorption was achieved with the addition of 0.0005 M AlCl 3 at pH 6.0. Adsorbed coliphages were eluted with 3% beef extract in Mcllvaine buffer at pH 7.1, with an average recovery of 78.74%. This concentration technique was applied for the detection of coliphages from the well water of Nagpur city (India) and found to contain coliphages in the range of 2 to 28 plaque‐forming units per liter (PFU/L).