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Multiplex Approach for Screening Genetic Markers of Microbial Indicators
Author(s) -
Stedtfeld Robert D.,
Baushke Sam,
Tourlousse Dieter,
Chai Benli,
Cole James R.,
Hashsham Syed A.
Publication year - 2007
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143007x181378
Subject(s) - multiplex , computational biology , dna microarray , biology , multiplex polymerase chain reaction , polymerase chain reaction , genetic marker , genetics , gene , gene expression
Genetic markers are expected to provide better specificity in epidemiological studies and potentially serve as better indicators of waterborne pathogens. Methods used to assess genetic markers of emerging microbial indicators include pulsed field gel electrophoresis, polymerase chain reaction (PCR), and microarrays. This paper outlines a high‐throughput approach to screen for such genetic markers using a set of theoretical and experimental screening tools. The theoretical screening involves evaluating genes related to the ribosomal RNA and specific functions from emerging indicator groups, followed by experimental validation with appropriate sampling schemes and high‐throughput and economical screening methods, such as microarrays, real time PCR, and on‐chip PCR. Analysis of a wide range of samples covering temporal variability in location, host, and waterborne disease outbreaks is essential. The proposed approach is expected to shorten the time and cost associated with searching for new genetic markers of emerging indicators by at least 10‐fold.