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2‐Chlorobenzoate Biodegradation by Recombinant Burkholderia cepacia under Hypoxic Conditions in a Membrane Bioreactor
Author(s) -
UrgunDemirtas Meltem,
Stark Benjamin,
Pagilla Krishna
Publication year - 2005
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143005x67421
Subject(s) - bioreactor , biodegradation , chemistry , chemical oxygen demand , burkholderia , microorganism , food science , biomass (ecology) , membrane bioreactor , bacterial growth , membrane reactor , microbiology and biotechnology , environmental chemistry , bacteria , membrane , environmental engineering , biochemistry , biology , sewage treatment , environmental science , organic chemistry , ecology , genetics
The feasibility of applying bacterial hemoglobin technology to degrade 2‐chlorobenzoate (2‐CBA) through co‐metabolism under hypoxic conditions in a membrane bioreactor (MBR) process has been studied in the laboratory. 2‐chlorobenzoate removal and chloride release rates in the MBR system varied from 99 to 78% and 98 to 73%, respectively, depending on the operation conditions. Chemical oxygen demand (COD) removal efficiencies were more than 90% at food‐to‐microorganism ratios ranging from 0.32 to 0.62 g/g/d, and the observed yield was 0.13 to 0.20 g biomass/g COD. The bacterial cell‐floc size‐distribution analysis showed that there is a significant change in bacterial floc size due to high shear stress during operation of the MBR. To characterize growth kinetics of Burkholderia cepacia strain dinitrotoluene, a mathematical model that describes co‐metabolic oxidation of 2‐CBA in an MBR has been developed.