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Substrate Inhibition Kinetics of Phenol Biodegradation
Author(s) -
Goudar Chetan T.,
Ganji Shobha H.,
Pujar Basayya G.,
Strevett Keith A.
Publication year - 2000
Publication title -
water environment research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 73
eISSN - 1554-7531
pISSN - 1061-4303
DOI - 10.2175/106143000x137103
Subject(s) - biodegradation , phenol , chemistry , substrate (aquarium) , kinetics , saturation (graph theory) , reaction rate constant , bacterial growth , chromatography , organic chemistry , bacteria , mathematics , ecology , biology , physics , genetics , quantum mechanics , combinatorics
Phenol biodegradation was studied in batch experiments using an acclimated inoculum and initial phenol concentrations ranging from 0.1 to 1.3 g/L. Phenol depletion and associated microbial growth were monitored over time to provide information that was used to estimate the kinetics of phenol biodegradation. Phenol inhibited biodegradation at high concentrations, and a generalized substrate inhibition model based on statistical thermodynamics was used to describe the dynamics of microbial growth in phenol. For experimental data obtained in this study, the generalized substrate inhibition model reduced to a form that is analogous to the Andrews equation, and the biokinetic parameters max , maximum specific growth; K s' , saturation constant; and K i' , inhibition constant were estimated as 0.251 h 2 1 , 0.011 g/L, and 0.348 g/L, respectively, using a nonlinear least squares technique. Given the wide variability in substrate inhibition models used to describe phenol biodegradation, an attempt was made to justify selection of a particular model based on theoretical considerations. Phenol biodegradation data from nine previously published studies were used in the generalized substrate inhibition model to determine the appropriate form of the substrate inhibition model. In all nine cases, the generalized substrate inhibition model reduced to a form analogous to the Andrews equation suggesting the suitability of the Andrews equation to describe phenol biodegradation data.