Open AccessSpectrofluorimetric Determination of Some N. Containing Medicines Using Rhodamine 6G as a Chromogenic Reagent
Author(s) -
Theia’a N. Al-Sabha,
Mohamed Y. Dhamra
Publication year - 2021
Publication title -
pakistan journal of analytical and environmental chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.155
H-Index - 3
eISSN - 2221-5255
pISSN - 1996-918X
DOI - 10.21743/pjaec/2021.12.15
Subject(s) - rhodamine 6g , reagent , chromogenic , famotidine , chemistry , chromatography , domperidone , calibration curve , fluorescence , rhodamine , detection limit , quenching (fluorescence) , pharmacology , organic chemistry , medicine , physics , quantum mechanics , molecule , dopamine , endocrinology
A sensitive spectrofluorimetric method has been developed for the analysis of some medicines containing primary, secondary, and tertiary amino groups, namely Diclofenac (DIC), Domperidone (DOM), Famotidine (FAM), and Propranolol (PRO), in their pure and medicinal forms. The method is based on the quenching of the fluorescence intensity of rhodamine 6G (R6G) through the formation of ion-pair complexes between the above medicines and the R-6G reagent, which is measured at 552 nm after excitation at 402 nm. The calibration graphs were rectilinear in the concentration ranges of 0.10- 9.00, 0.05-15.00, 0.10-14.0 and 0.05-5.00 µg mL-1 for above medicines respectively. The recovery (%) values were ranged between 99.45%- 100.97%. The detection limits ranged in the concentration of 0.243-0.754 µg/mL, and the limits of quantitation were 0.806- 2.420 µgmL-1 for all drugs. The method was successfully applied for the determination of these drugs in their pharmaceutical preparations.