Open AccessEnvironmental Friendly Production of Amylase from Aspergillus niger EFRL-FC-024 Using Corn Waste as Carbon Source
Author(s) -
Abdul-Nabi Mirjatt,
Imrana Khushk,
Hiba Zahid,
Abdul Sattar Qureshi,
Muhammad Aqeel Bhutto,
Bushra Watoor
Publication year - 2021
Publication title -
pakistan journal of analytical and environmental chemistry
Language(s) - English
Resource type - Journals
eISSN - 2221-5255
pISSN - 1996-918X
DOI - 10.21743/pjaec/2021.06.17
Subject(s) - aspergillus niger , amylase , bagasse , food science , starch , chemistry , microorganism , fermentation , enzyme , agronomy , biology , microbiology and biotechnology , biochemistry , bacteria , genetics
Amylase is an indispensable and industrially important enzyme that hydrolyzes carbohydrates particularly starch into simple sugars. Amylase enzymes have been isolated from various sources such as microbes, animals and plants. However, microorganisms are highly preferred as compared to plants and animal sources. Amylases of fungal origin are highly stable compared to amylases produced by bacterial species. The aim of this study was to investigate the production of extracellular amylase enzyme from Aspergillus niger EFRL-FC-024 using sugarcane bagasse and corn waste as an energy source under submerge fermentation conditions. Primarily, the fungal strain was grown for 6 days using sugarcane bagasse and corn waste, respectively. Mainly, the growth of a microorganism was also evaluated using different pH, temperature and incubation periods. The results revealed maximum amylase production of 1.64 U/mL when A. niger was cultured for 96 h using corn waste. Moreover, addition of different nitrogen sources showed the highest amylase production when peptone was supplemented as a nitrogen source. Finally, the effect of pH indicated maximal concentration of amylase enzyme at pH 6.0. The present study will highly be beneficial to explore the role of fungal strain A. niger in amylase production at Industrial levels.