Open AccessRatiometric and Selective Fluorescent Nanosensor for the Detection of Glucose
Author(s) -
Shengda Qi
Publication year - 2021
Publication title -
current chinese chemistry
Language(s) - English
Resource type - Journals
eISSN - 2666-0016
pISSN - 2666-0008
DOI - 10.2174/2666001601666210106121954
Subject(s) - nanosensor , fluorescence , glucose oxidase , photochemistry , detection limit , quenching (fluorescence) , quantum dot , chemistry , biosensor , hydrogen peroxide , analytical chemistry (journal) , materials science , nanotechnology , chromatography , biochemistry , optics , physics
Background and Methods: Dual-emission fluorescent nanosensor for the detection ofglucose has been developed by simply mixing the blue-emission C-dots (CDs) with red-emissionglutathione modified CdTe QDs. Results and Discussion: The ratiometric fluorescence system exhibits dual emission peaks at 442nm and 600 nm under a single excitation wavelength (365 nm). Due to the strong quenching effectof hydrogen peroxide to the quantum dots, the fluorescence of the QDs in the nanocompositesystem could be selectively quenched by H2O2, while the fluorescence of the CDs remainedconstant, resulting in a distinguishable fluorescence color evolution (from red to blue) of thenanosensor which could be visible through naked eye. When this nanosensor was mixed withglucose oxidase, H2O2 can be produced by the enzyme catalysis reaction, which could quench thefluorescent intensity of QDs. So this system can be used to detect glucose. The limit of detection ofthis present method was as low as 0.1 μM. Conclusion: The result for glucose was satisfying, suggesting its potential application for glucosesensing.