Open AccessOptimization of Metabolite Extraction Protocols for the Identification and Profiling of Small Molecule Metabolites from Planktonic and Biofilm Pseudomonas aeruginosa Cultures
Author(s) -
Amanda L. Fuchs,
Brian Tripet,
Mary Cloud B. Ammons,
Valérie Copié
Publication year - 2016
Publication title -
current metabolomics
Language(s) - English
Resource type - Journals
eISSN - 2213-2368
pISSN - 2213-235X
DOI - 10.2174/2213235x04666151126203043
Subject(s) - pseudomonas aeruginosa , biofilm , metabolite , microbiology and biotechnology , metabolite profiling , profiling (computer programming) , identification (biology) , metabolomics , chemistry , computational biology , biology , bacteria , chromatography , biochemistry , genetics , computer science , botany , operating system
Metabolomics aims to characterize the metabolic phenotype and metabolic pathways utilized by microorganisms or other cellular systems. A crucial component to metabolomics research as it applies to microbial metabolism is the development of robust and reproducible methods for extraction of intracellular metabolites. The goal is to extract all metabolites in a non-biased and consistent manner; however, most methods used thus far are targeted to specific metabolite classes and use harsh conditions that may contribute to metabolite degradation. Metabolite extraction methodologies need to be optimized for each microorganism of interest due to different cellular characteristics contributing to lysis resistance.