Open AccessEpidermal Growth Factor rs4444903 A>G Gene Polymorphism Association with Chronic Hepatitis B Liver Disease Progression among Egyptian Children: A Multicenter Study
Author(s) -
Amal Ahmed Mohamed,
Gehan Lotfy Abdel Hakeem,
Gihan M. Babrs,
Laila E. Abolfotoh,
Nageh M. Shehata,
Sheren Esam Maher,
Suzan Omar Mousa,
Ahlam M. Ismail,
Doaa Ezzat,
Ahmed G. K. Habib,
Doaa Ghaith,
Naglaa Fawazy,
Rania Abdelmonem Khattab,
Eman El S. Habib,
Dalia M. Abd El-Hassib,
Sherief Abd-Elsalam,
Dalia A. El-damasy
Publication year - 2021
Publication title -
the open biomarkers journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.184
H-Index - 5
ISSN - 1875-3183
DOI - 10.2174/1875318302111010063
Subject(s) - genotype , medicine , allele , hepatitis b virus , gastroenterology , allele frequency , immunology , polymorphism (computer science) , epidermal growth factor , liver disease , single nucleotide polymorphism , gene polymorphism , cirrhosis , virus , gene , biology , genetics , receptor
Background: Polymorphisms of genes encoding the pro-inflammatory and anti-inflammatory cytokines can affect the clinical presentation of the infection. We aimed to assess the role of EGF gene single-nucleotide polymorphism in the outcome of chronic hepatitis B virus (HBV) infection in children. Methods: One hundred HBV-infected children and 75 healthy matched controls were enrolled in this prospective study. Patients included 18 chronic inactive and 82 chronic active carriers. EGF rs4444903 A>G genotypes were determined using allele-specific amplification. Results: Significant differences regarding EGF genotypic frequency ( p =0.001) in patients compared to controls ( p =0.001). Eighteen percent were inactive, and 82% were active carriers. AA, AG and GG genotypic frequency were 66.7%, 33.3%, 0% and were 3.7%, 37.8% and 58.5% in the inactive and active carriers, respectively, with significant differences regarding AA, AG, GG genotypic frequency ( p =0.001 for all). EGF AA, AG, GG genotypes frequency were 1.9%, 33.3%, and 64.8%, respectively, with significant differences between cirrhotic and non-cirrhotic patients regarding AA, AG, GG genotypic frequency ( p =0.001 for all). Conclusion: Increased G allele frequency in EGF rs4444903 A > G polymorphism in HBV- Egyptian children is associated with worse outcomes.