Open AccessJustification of Traditional Uses of Asparagus Racemosus (Shatavari) - A Miracle Herb
Author(s) -
Samira Karim,
Md. Shariful Islam,
Zarin Tasnim,
Farhina Rahman Laboni,
Azima Sultana Julie,
Zubair Khalid Labu
Publication year - 2017
Publication title -
the open bioactive compounds journal
Language(s) - English
Resource type - Journals
ISSN - 1874-8473
DOI - 10.2174/1874847301705010009
Subject(s) - chemistry , petroleum ether , chloroform , asparagus racemosus , dpph , antioxidant , lysis , carbon tetrachloride , chromatography , antimicrobial , ascorbic acid , food science , traditional medicine , biochemistry , medicinal plants , organic chemistry , extraction (chemistry) , medicine
Objective: The ethanolic plant extracts of Asparagus racemosus and their partitionates were assessed for thrombolytic, membrane stabilizing, antimicrobial and antioxidant activity in vitro . Methods: The collected plant was dried and grinded. The coarse powder was shocked in 2000 mL of 90% methanol for several days then filtrated. At 40°C the volume of crude ethanolic extract (CEE) was reduced by a vacuum rotary evaporator followed by concentrated aqueous ethanol extractive was fractionated into petroleum ether (PSF), carbon tetrachloride (CTSF), chloroform soluble fraction (CSF) and aqueous (AQSF) soluble fractions. Results: In thrombolytic assay among all the extractives, carbon tetrachloride soluble fraction (CTSF) showed the highest clot lysis (41.48 ± 0.43%) activity as compared with standard streptokinase (65.16 ± 0.16%). With respect to the membrane stabilizing activity, chloroform soluble fraction (CSF) profoundly inhibited the lysis of erythrocytes (80.86 ± 0.36%) induced by osmosis, whereas reference standard acetyl salicylic acid (ASA) resulted in (88.99 ± 0.39%) inhibition of lysis. On the other hand, CTSF showed slightly higher level of protection against heat-induced hemolysis (85.68 ± 0.75%) as same as with ASA, (85.68 ± 0.75%)). In antimicrobial investigation, crude ethanol extract (CEE) and their partitioning fractions exhibited moderate inhibition of growth of some examined bacteria. The total phenolic content was found maximum (930.94 mg of GAE/gm) in CEE of A. racemosus. The antioxidant potentials in term of DPPH free radical scavenging assay showed EC50 values 2.62 μg/mL by aqueous soluble fraction (AQSF) in comparison to ascorbic acid 2.48 μg/mL as a reference standard. Conclusions: This study was conducted to validate the A. racemosus plant as thrombolytic, membrane stabilizing agent, antimicrobial and antioxidant.