Open AccessPlant Growth-promoting and Bio-control Activity of Micrococcus luteus Strain AKAD 3-5 Isolated from the Soybean (Glycine max (L.) Merr.) Rhizosphere
Author(s) -
Anamika Dubey,
Ashwani Kumar,
Mohammed Latif Khan,
Devendra Payasi
Publication year - 2021
Publication title -
the open microbiology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.502
H-Index - 27
ISSN - 1874-2858
DOI - 10.2174/1874285802115010188
Subject(s) - rhizosphere , biology , micrococcus luteus , biofertilizer , rhizobacteria , cellulase , botany , bacteria , food science , horticulture , microbiology and biotechnology , biochemistry , enzyme , staphylococcus aureus , genetics
Background: Applications of bioinoculants for improving crop productivity may be an eco-friendly alternative to chemical fertilizers. Rhizosphere or soil-inhabiting beneficial microbes can enhance plant growth and productivity through direct and indirect mechanisms, i.e. , phosphate solubilization, nutrient acquisition, phytohormone production, etc. Objective: This study is based on the hypothesis that diseases resistant plants can act as a source of potential microbes that can have good plant growth-promoting traits and bio-control potential. Methods: In this study, we have isolated the rhizobacterial strains (AKAD 2-1, AKAD 2-10, AKAD 3-5, AKAD 3-9) from the rhizosphere of a disease-resistant variety of soybean (JS-20-34) ( Glycine max (L.) Merr.). These bacterial strains were further screened for various plant growth-promoting traits (phosphate solubilization, indole acetic acid (IAA), ammonia, biofilm, HCN, Exopolysaccharide (EPS), and enzyme production activity (catalase, cellulase, and chitinase)). Results: Among four, only bacterial strain AKAD 3-5 has shown plant-growth-promoting and biocontrol (98%) activity against Fusarium oxysporum . Morphological, biochemical, and molecular characterization (16S rRNA) revealed that this rhizobacterial isolate AKAD 3-5 closely resembles Micrococcus luteus (Gene bank accession: MH304279). Conclusion: Here, we conclude that this strain can be utilized to promote soybean growth under varied soil stress conditions.