Open AccessStudy of Various Virulence Genes, Biofilm Formation and Extended-Spectrum β-lactamase Resistance in Klebsiella pneumoniae Isolated from Urinary Tract Infections
Author(s) -
Sanaa S. Hamam,
Reem M El Kholy,
Magdi E. A. Zaki
Publication year - 2019
Publication title -
the open microbiology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.502
H-Index - 27
ISSN - 1874-2858
DOI - 10.2174/1874285801913010249
Subject(s) - microbiology and biotechnology , klebsiella pneumoniae , aerobactin , virulence , biology , biofilm , imipenem , amikacin , ceftazidime , gene , antibiotic resistance , bacteria , antibiotics , enterobacteriaceae , escherichia coli , pseudomonas aeruginosa , genetics
Objective: The aims of the current study were to evaluate the capacity of K. pneumoniae isolated from hospital-acquired urinary tract infection to form biofilm, the relation of this capacity to various virulence genes and the prevalence of Extended Spectrum β-lactamases (ESBL) among these isolates by phenotypic and genotypic methods. Material and Methods: The study included 100 non-duplicate strains of K. pneumoniae isolated from 100 different urine samples from patients with hospital-acquired urinary tract infection. The isolated strains were studied for biofilm formation, ESBL production by phenotypic methods. Molecular studies were applied for the detection of ESβLs genes bla TEM , bla SHV , bla CTX - M and for detection of virulence genes fim H, uge, rmp A, mag A, wzy, kfa and aerobactin genes. Result: The majority of the isolates had the capacity to form a biofilm (81%), with ESBL prevalence rate 41%. The most prevalent gene among ESBL producing K. pneumoniae was bla CTX-M (73.2%) followed by bla SHV (53.6%) and bla TEM (51.2%). Among the virulence genes studied in K. pneumoniae isolates, the most prevalent gene was fimH (76%), uge (70%). There was significant association between ESBL production, and resistance to amikacin, cefepime, ceftazidime, gentamicin, imipenem and meropenem and biofilm production in K. pneumoniae isolates. There was significant association between bla CTX-M , bla SHV , fim H, mag , kfa , wzy, rmp A and aerobactin and biofilm production in K. pneumoniae . Conclusion: The present study highlights the prevalence of virulence genes among biofilm-forming strains of K. pneumoniae isolated from hospital-acquired urinary tract infection. Moreover, there was association between biofilm formation and ESBL production. Further studies are required to elucidate the clinical impact of the association of these different mechanisms.