Open AccessInfluence of Nitrogen Source on 2,4-diacetylphloroglucinol Production by the Biocontrol Strain Pf-5
Author(s) -
Malin Hultberg,
Beatrix Alsanius
Publication year - 2008
Publication title -
the open microbiology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.502
H-Index - 27
ISSN - 1874-2858
DOI - 10.2174/1874285800802010074
Subject(s) - pseudomonas fluorescens , nitrogen , ammonium , nitrate , nutrient , ammonium nitrate , strain (injury) , biology , chemistry , sodium nitrate , food science , microbiology and biotechnology , botany , bacteria , inorganic chemistry , ecology , organic chemistry , anatomy , genetics
The production of 2,4-diacetylphloroglucinol (DAPG) by the biocontrol agent Pseudomonas fluorescens Pf-5 was studied in nutrient-solution based media with varying nitrogen content. No production of DAPG was observed when organic nitrogen was omitted from the media, regardless of the inorganic nitrogen source used. Furthermore, a micromolar concentration range of organic nitrogen was insufficient to sustain production. When a millimolar concentration range of organic nitrogen was used, DAPG production was observed in the medium with ammonium as the inorganic nitrogen source. No production was observed in the treatments with ammonium and nitrate or nitrate only, despite growth of the bacterial strain being the same for all treatments. These results suggest that it is possible to manipulate the nutrient solution to increase the reliability and efficacy of biological control agents.