Open AccessMolecular Study of Klebsiella Oxytoca Associated with Urinary Tract Infection in Children
Author(s) -
Raghdaa Shrief,
Rasha Hassan,
Maysaa El Sayed Zaki,
Mohamed A. Rizk
Publication year - 2022
Publication title -
the open microbiology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.502
H-Index - 27
ISSN - 1874-2858
DOI - 10.2174/18742858-v16-e2201070
Subject(s) - klebsiella oxytoca , microbiology and biotechnology , bacterial adhesin , biology , biofilm , gene , bacteria , enterobacteriaceae , escherichia coli , genetics
Background: There are scarce reports about the association of Klebsiella oxytoca ( K. oxytoca ) with urinary tract infection (UTI) in children. We aimed to evaluate the prevalence of fimA, mrkA, matB and pilQ adhesins genes and extended-spectrum beta-lactamase (ESBL) genes blaCTX-M, blaTEM and blaSHV by polymerase chain reaction (PCR) and to study biofilm formation and antibiotics resistance in K. oxytoca from children with UTI. Methods: This study was a retrospective cross-sectional study that included 120 children with UTI due to K. oxytoca . The bacteria were subjected to molecular detection of fimA, mrkA, matB and pilQ adhesins genes and ESBL genes blaCTX-M, blaTEM and blaSHV by PCR. Biofilm capacity was determined by the microtiter plate method. Results: The isolated K. oxytoca had positive ESBL activity in 45.8% of isolates. About 40% of isolates were biofilm producers. The frequency of adhesion genes among K. oxytoca was 91.7%, 83.3%, 48.3% and 37.5% for matB, pilQ, fimA and mrkA genes, respectively. For ESBL genes, the frequency was 38.3%, 36.7% and 33.3% for blaCTX-M, blaSHV and blaTEM genes, respectively. The commonest genes among ESBL isolates were blaCTX-M (83.6%), blaSHV (80%) then blaTEM gene (72.7%). A significant association (p=0.048) was detected between ESBL activity and biofilm formation by K. oxytoca. Conclusion: Present study highlights the emergence of K. oxytoca as a pathogen associated with UTI in children. There was a high prevalence of adhesin genes and ESBL genes among these isolates. The capacity of K. oxytoca to form biofilm was associated with ESBL production.