Open AccessAnalysis Fatty Acids Profile in Tabanus bivittatus Mats with Gas Chromatography-Mass Spectrometry
Author(s) -
Yanhua Wang,
Fuhua Wu,
Guo Zhaohan,
Mingxing Peng,
Min Xia,
Pang Zhen-ling,
Xiaoli Wang,
Zian Liang,
Zhang Naiqun
Publication year - 2015
Publication title -
the open biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 11
ISSN - 1874-0707
DOI - 10.2174/1874070701509010113
Subject(s) - oleic acid , stearic acid , fatty acid , linoleic acid , palmitic acid , extraction (chemistry) , chromatography , biology , chemistry , biochemistry , organic chemistry
Tabanus bivittatus Mats., a traditional Chinese medicine, is commonly used for cardiovascular disorders treatment including atherosclerosis. There have been only a few researches on its chemical components, and no detailed report has appeared on its fatty acids. To develop a simple and effective method for the extraction of total fatty acids from Tabanus bivittatus Mats., the Soxhlet extraction (SE) condition was optimized with response surface methodology. The fatty acid composition of the extract were determined by GC-MS with previous derivatization to fatty acid methyl esters (FAMEs). The major fatty acids in Tabanus bivittatus Mats. were oleic acid, palmitic acid, linoleic acid, palmitoleic acid, and stearic acid, and the unsaturated fatty acids occupy 63.9% of the total fatty acids.