Open AccessFuranocoumarin A: A Novel Anticancer Agent on Human Lung Cancer A549 Cells from Fructus liquidambaris
Author(s) -
Hui Fang,
Hongmei Ji
Publication year - 2020
Publication title -
anti-cancer agents in medicinal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.508
H-Index - 96
eISSN - 1875-5992
pISSN - 1871-5206
DOI - 10.2174/1871520619666191010102526
Subject(s) - furanocoumarin , lung cancer , a549 cell , human lung , pharmacology , traditional medicine , chemistry , medicine , lung , oncology , biochemistry
Background and Purpose: The fruit of Fructus liquidambaris, which is recently being used forcancer treatment, has a history to be used as a traditional medicine in China for thousands of years. Material and Methods: Ten kg of dried F. liquidambaris was obtained with 70% alcohol-water solution underreflux for three times. The condensed extract was obtained from petroleum ether, ethyl acetate and N-butylalcohol, respectively. Ethyl acetate extract was subjected to silica gel column, Sephadex LH-20, ODS columnchromatography and RP-HPLC column chromatography to yield a new compound (1). The structure was identifiedthrough intensive analysis of NMR and MS spectra. The antitumor mechanism of the furanocoumarin A onhuman lung cancer A549 cells was confirmed by detecting the apoptosis-related proteins. Result: Furanocoumarin A (1), a novel furanocoumarin constituent was isolated and identified from F. Liquidambaris.The IC50 value of furanocoumarin A on A549 cell lines was 65.28±5.36µM obtained by the method ofMTT. The compound could induce the apoptosis of A549 cells by inducing 21.5% early apoptosis and 32.4%late apoptosis at the concentration of 60µmol/L. Western blot analysis indicated that protein expressions of p53,caspase 3 and Bax increased in a dose-dependent manner between the concentrations from 40 to 80µM. Theprotein expression of Bcl-2 decreased the concentration of 60 and 80&3181;M. The ratio of Bcl-2 to Bax was inverselyproportional to the dose concentration. Conclusion: Furanocoumarin A could be a novel anticancer agent from herbal medicine.