Open AccessNew Metabolites of Udenafil Identified through Liquid Chromatography– Quadrupole Time-of-flight Mass Spectrometry
Author(s) -
Jae Sung Pyo
Publication year - 2021
Publication title -
current pharmaceutical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 24
eISSN - 1875-676X
pISSN - 1573-4129
DOI - 10.2174/1573412917666201208114954
Subject(s) - chemistry , chromatography , metabolite , mass spectrometry , in vivo , liquid chromatography–mass spectrometry , tandem mass spectrometry , extraction (chemistry) , urine , solid phase extraction , biochemistry , microbiology and biotechnology , biology
Background: Udenafil, a recently discovered drug used for erectile dysfunction treatment, has been widelyprescribed and its effect on human systems has been extensively studied. However, there is little research on the humanmetabolites of udenafil. Three metabolites have been identified in rats. Objective: Herein, highly sensitive and accurate liquid chromatography–quadrupole time-of-flight tandem massspectrometry (LC-Q-TOF-MS/MS) was conducted to identify new udenafil metabolites. Methods: Human liver microsomes were incubated with udenafil for in vitro samples, and rat urine and faeces sampleswere collected from udenafil-administered rats for in vivo samples. Each sample was deproteinated with acetonitrile andextracted by solid phase extraction. The purified samples were separated and analyzed by LC-Q-TOF-MS, and somemetabolite candidates were reanalyzed for further structural analysis using LC-Q-TOF-MS/MS. Results: Eleven and three metabolites were identified in the in vitro and in vivo samples, respectively, and were found tobe hydrolyzed, oxidized, or demethylated forms of udenafil or its metabolites. The error of the metabolic analysis was−8.7 to 7.6 ppm, indicating the high accuracy of the method. Conclusion: These metabolic results could be useful for further investigation of udenafil and new phosphodiesterase-5inhibitors.