Open AccessEtlingera elatior (Jack) R.M, Sm Containing Diet Normalizes Some Metabolic Syndrome Markers due to High-fat High-fructose Diet in Wistar Rats
Author(s) -
I.D. Hanifah Nur,
Retno Murwani,
Achmad Zulfa Juniarto
Publication year - 2021
Publication title -
current nutrition and food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.221
H-Index - 25
eISSN - 2212-3881
pISSN - 1573-4013
DOI - 10.2174/1573401316666201208101359
Subject(s) - fructose , malondialdehyde , metabolic syndrome , post hoc , phytochemical , food science , analysis of variance , obesity , medicine , biology , zoology , oxidative stress , traditional medicine
Background: Etlingera elatior (Ee) contains phytochemical compounds that are rich inantioxidants, which may reduce several biochemical markers of metabolic syndrome (MetS). Objective: We aimed to study the effect of fresh Etlingera elatior (FEe) and steamed Etlingera elatior(SEe) as a part of rat diet on body weight, serum lipid, and malondialdehyde (MDA) level inWistar rats with MetS induced by a high-fat, high-fructose diet. Methods: Our research was a true experimental randomized control group design with pre- andpost-test. A total of 24 male Wistar rats were divided randomly into the following four groups: 1)Control, fed standard rat diet during the whole duration of the study, 2) HFFr-Sd, fed high-fat high--fructose (HFFr) diet for 29 days, followed by 29 days of the standard diet, 3) HFFr-FEe, fed HFFrdiet for 29 days, followed by 29 days of a standard diet containing 33.3% FEe, and 4) HFFr-SEe,fed HFFr diet for 29 days, followed by 29 days of a standard diet containing 33.3% SEe. The HFFrdiet was given at 15 g/day along with fructose drink (20% pure fructose) at 100 ml/day. The dietsin each group after the MetS induction period are referred to as intervention diets. Data at the endof HFFr (pre) and intervention diets (post) were analyzed by paired t-test. The data among groupswere analyzed by one-way analysis of variance followed by post hoc test. Results: HFFr diet for 29 days induced MetS in Wistar rats fulfilling the criteria of obesity (Lee Index),hypertriglyceridemia, and decreased high-density lipoprotein cholesterol (HDL-C). Also,there was a significant increase in serum total cholesterol, low-density lipoprotein cholesterol(LDL-C), and MDA level (p < 0.05). Feeding a diet containing FEe or SEe can significantly reducebody weight, serum triglyceride, total cholesterol, LDL-C, and MDA, and increase HDL-C levels(p < 0.05). The effect of FEe was more pronounced in ameliorating body weight and lipid profilethan SEe. Conclusion: Fresh Ee and Steamed Ee can ameliorate obesity, dyslipidemia, and oxidative stressin MetS Wistar rats induced by a high-fat, high-fructose diet. It suggests that dietary Ee accountingfor one-third of daily standard diet can assist in normalizing some MetS markers in rats.