Open AccessMethylation Profile of miR-9-1 and miR-9-1/-9-3 as Potential Biomarkers of Diabetic Retinopathy
Author(s) -
Caroline Severo de Assis,
Alexandre Silva,
Mayara Karla dos Santos Nunes,
João Aragão Ximenes Filho,
Rayner Anderson Ferreira do Nascimento,
Cecília Neta Alves Pegado Gomes,
Isabella Evangelista,
Naila Francis Paulo de Oliveira,
Darlene Camati Persuhn
Publication year - 2021
Publication title -
current diabetes reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.631
H-Index - 57
eISSN - 1875-6417
pISSN - 1573-3998
DOI - 10.2174/1573399817666210101104326
Subject(s) - diabetic retinopathy , medicine , diabetes mellitus , methylation , retinopathy , dna methylation , population , microrna , endocrinology , bioinformatics , biology , gene , gene expression , genetics , environmental health
Aims: Analysis of the relationship between the methylation profile of miR-9-1 or miRs-9-1 / -9-3 and diabetic retinopathy. Background: Diabetic Retinopathy (DR) is a frequent complication of Diabetes mellitus and it hasa decisive impact on the quality of life, as it is one of the biggest causes of blindness in the adultpopulation. Levels of microRNA-9 have been shown to be related to diabetes but little is knownabout its involvement with DR in humans. Objective: To analyze the relationship between the methylation profile of miR-9-1 or miRs-9-1/-9-3 and DR. Methods: 103 patients diagnosed with diabetes for 5 to 10 years were analyzed. The data were categorizedaccording to clinical, biochemical, lifestyle and anthropometric parameters. DNA extractedfrom leukocyte samples was used to determine the methylation profile of miRs-9-1 and -9-3 usinga specific methylation PCR assay. Results: miR-9-1 methylation was related to diabetic retinopathy, indicating that methylation ofthis miR increases the chances of presenting retinopathy up to 5 times. In our analyses, diabeticswith lower levels of creatinine and CRP showed significant reductions (99% and 97%) in presentingDR. Methylation of both miRs-9-1 and 9-3 methylated increases the chances of presenting DRby 8 times; in addition, a sedentary lifestyle can increase the risk for the same complication by upto 6 times. Conclusion: Our results suggest that both methylation of miR-9-1 and e miRs-9-1 / 9-3 favors DRin patients with diabetes in a period of 5 to 10 years of diagnosis.