Open AccessTranscriptome Analysis of FEN1 Knockdown HEK293T Cell Strain Reveals Alteration in Nucleic Acid Metabolism, Virus Infection, Cell Morphogenesis and Cancer Development
Author(s) -
SongBai Liu,
Xiu-Qin Qiu,
Weiqiang Guo,
Jinli Li,
Qian Su,
Jiahui Du,
HeJuan Hu,
Xiaoxiao Wang,
YaoHua Song,
Ling Xiao,
Xiangbin Xu
Publication year - 2019
Publication title -
combinatorial chemistry and high throughput screening
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.292
H-Index - 64
eISSN - 1875-5402
pISSN - 1386-2073
DOI - 10.2174/1386207322666190704095602
Subject(s) - biology , ribosome biogenesis , gene knockdown , cell cycle , rna , microbiology and biotechnology , cell growth , cell , dna repair , dna , ribosome , cell culture , genetics , gene
Aim and Objective: Flap endonuclease-1 (FEN1) plays a central role in DNA replication andDNA damage repair process. In mammals, FEN1 functional sites variation is related to cancer and chronicinflammation, and supports the role of FEN1 as a tumor suppressor. However, FEN1 is overexpressed inmultiple types of cancer cells and is associated with drug resistance, supporting its role as an oncogene.Hence, it is vital to explore the multi-functions of FEN1 in normal cell metabolic process. This study wasundertaken to examine how the gene expression profile changes when FEN1 is downregulated in 293Tcells. Materials and Methods: Using the RNA sequencing and real-time PCR approaches, the transcriptexpression profile of FEN1 knockdown HEK293T cells have been detected for the next step evaluation,analyzation, and validation. Results: Our results confirmed that FEN1 is important for cell viability. We showed that when FEN1downregulation led to the interruption of nucleic acids related metabolisms, cell cycle related metabolismsare significantly interrupted. FEN1 may also participate in non-coding RNA processing, ribosome RNAprocessing, transfer RNA processing, ribosome biogenesis, virus infection and cell morphogenesis. Conclusion: These findings provide insight into how FEN1 nuclease might regulate a wide variety ofbiological processes, and laid the foundation for understanding the role of other RAD2 family nucleases incell growth and metabolism.