Open AccessBoosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives
Author(s) -
Nariyasu Tahara,
Itaru Tachibana,
Kazuyo Takeo,
Shinji Yamashita,
Atsuhiro Shimada,
Misuzu Hashimoto,
Satoshi Ohno,
Takashi Yokogawa,
Tsutomu Nakagawa,
Fujio Suzuki,
Akio Ebihara
Publication year - 2021
Publication title -
protein and peptide letters/protein and peptide letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 58
eISSN - 1875-5305
pISSN - 0929-8665
DOI - 10.2174/0929866528666210805120715
Subject(s) - recombinant dna , escherichia coli , lactose , inducer , lac operon , thermus thermophilus , biochemistry , chemistry , biology , gene
Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems. Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates. The differences in culture substrates sometimes lead to variations in the induction level.