Open AccessQuantification of Angiogenesis and Lymphangiogenesis in the Dual ex vivo Aortic and Thoracic Duct Assay
Author(s) -
Shuangyong Wang,
Michael Yamakawa,
Samuel M. Santosa,
Neeraj Chawla,
Kai Guo,
Mario Montana,
Joelle Hallak,
Kyu-Yeon Han,
Masatsugu Ema,
Mark I. Rosenblatt,
Jin-Hong Chang,
Dimitri T. Azar
Publication year - 2019
Publication title -
protein and peptide letters/protein and peptide letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 58
eISSN - 1875-5305
pISSN - 0929-8665
DOI - 10.2174/0929866526666190925145842
Subject(s) - lymphangiogenesis , thoracic duct , ex vivo , angiogenesis , lymphatic system , lymphatic endothelium , pathology , lymphatic vessel , vascular endothelial growth factor c , thoracic aorta , chylothorax , aorta , vascular endothelial growth factor , biology , medicine , microbiology and biotechnology , in vivo , cancer research , metastasis , vascular endothelial growth factor a , vegf receptors , cancer
Lymphatic vessel formation (lymphangiogenesis) plays important roles in cancer metastasis, organ rejection, and lymphedema, but the underlying molecular events remain unclear. Furthermore, despite significant overlap in the molecular families involved in angiogenesis and lymphangiogenesis, little is known about the crosstalk between these processes. The ex vivo aortic ring assay and lymphatic ring assay have enabled detailed studies of vessel sprouting, but harvesting and imaging clear thoracic duct samples remain challenging. Here we present a modified ex vivo dual aortic ring and thoracic duct assay using tissues from dual fluorescence reporter Prox1- GFP/Flt1-DsRed (PGFD) mice, which permit simultaneous visualization of blood and lymphatic endothelial cells.