Open AccessAnaerobic Fe(III) reduction by Shewanella putrefaciens: Analysis of the electron transport chain
Author(s) -
Daâd A. Saffarini
Publication year - 2004
Language(s) - English
Resource type - Reports
DOI - 10.2172/820811
Subject(s) - shewanella oneidensis , shewanella , shewanella putrefaciens , mutant , electron transport chain , chemistry , reduction (mathematics) , biochemistry , computational biology , microbiology and biotechnology , bacteria , biology , gene , genetics , geometry , mathematics
The goals of the project were to isolate mutants that are deficient in metal reduction, identify components of the electron transport chain that are involved in this process, and purify some of these proteins for biochemical analyses. In the 3-year period since the start of the project, we have accomplished many of these goals. We have isolated several new S. oneidensis mutants that are deficient in metal reduction, and have initiated the development of vectors for the overexpression of cytochromes and other proteins in S. oneidensis. We have also overexpressed CymA, one of the c cytochromes that are involved in metal reduction