Open AccessManual collapse of blastocoels in not effective in increasing the viability of vitrified equine embryos
Author(s) -
Andrez Pastorello Bohn,
Arnaldo Diniz Vieira,
Thomaz Lucia,
Bernardo Garziera Gasperin,
Rafael Gianella Mondadori
Publication year - 2022
Publication title -
acta veterinaria brasilica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 8
ISSN - 1981-5484
DOI - 10.21708/avb.2022.16.1.10311
Subject(s) - vitrification , cryopreservation , blastocoel , andrology , embryo , embryo transfer , ovulation , zygote , biology , gynecology , chemistry , pregnancy , medicine , embryogenesis , blastocyst , genetics
Embryo cryopreservation methods have been used for commercialization and formation of genetic banks. Cryopreservation of equine embryos 300 μm in diameter, needing blastocoel collapse for satisfactory resistance to cryopreservation by vitrification. To evaluate the viability of simplified blastocoel collapse by embryo puncture with low technology and low-cost equipment, 22 embryos, collected at day 8 post-ovulation (D8), were allocated to the following groups: (1) micropuncture with a 30 G needle, assisted by a mechanical micromanipulator, before vitrification (n=4); (2) manual blade microsection before vitrification (n=6); (3) no manipulation prior to vitrification (n=8); and (4) freshly inovulated embryos (n=4). Despite the high re-expansion rates observed after vitrification, embryos manipulated prior to vitrification (groups MP and MS) did not result in pregnancy 25 days after transfer. On the other hand, embryos from groups NM (non-micromanipulated) and FR (freshly inovulated) resulted in pregnancies at 25 days. Under the conditions of the present study, manual blastocoel collapse was not efficient in increasing cryotolerance to vitrification among large embryos, requiring improvements to obtain pregnancies.