Open AccessTherapeutic laser with or without andiroba oil in treating cutaneous wounds by second intention in Wistar rats
Author(s) -
Rômulo Silva de Oliveira,
Mayara Marques Pereira Fernandes,
Millena do Nascimento Mesquita,
Ana Caroline Lima da Cruz,
Charles Pelizzari,
Eduardo Cavalcante das Neves,
Rui Carlos Peruquetti,
Marina Maurente Berón,
Aline de Marco Viott,
Soraia Figueiredo de Souza
Publication year - 2021
Publication title -
acta veterinaria brasilica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 8
ISSN - 1981-5484
DOI - 10.21708/avb.2021.15.4.10027
Subject(s) - medicine , granulation tissue , saline , edema , therapeutic effect , wound healing , surgery , necrosis , dermatology , pathology , anesthesia
This study’s objective was to evaluate the efficacy of treating cutaneous wounds in Wistar rats using a therapeutic laser alone or in combination with topical application of andiroba oil. Twenty-four Wistar rats were distributed into three groups (T4, T7, and T14) of eight animals. To prepare the wounds, a total of four skin fragments per animal were removed using an 8-mm cutaneous biopsy punch. Each animal was inflicted with four surgical wounds, and each wound was subjected to one treatment. The treatments were as follows: saline solution (control, Cn); laser therapy (L), using a 660-nm laser wavelength and 10-J/cm² energy density; fresh andiroba oil (An); laser therapy followed by topical andiroba oil administration (LAn). All treatments in all animals were conducted for 4- (T4), 7- (T7), and 14- (T14) day periods. Edema and purulent secretion were observed in three animals in the An group, and the appearance of an exuberant crust was also observed in one animal from the same group. The LAn group presented the worst wound healing rate and contraction velocity (p < 0.05). Microscopically, there was no difference between groups regarding the presence of inflammation, necrosis, formation of granulation tissue, fibroplasia, and the presence of types 1 and 3 collagen at different treatment times. It was concluded that laser treatment of cutaneous wounds in conjunction with andiroba oil application did not present benefits in reference to the 0.9% NaCl.