Open AccessImmunolocalization of angiotensin-(1-7), angiotensin II and angiotensin-converting enzyme 2 in goat ovary
Author(s) -
Lauro César Soares Feitosa,
Alécio Matos Pereira,
Adonias Almeida Carvalho,
Artemízia Francisca de Sousa,
Kinulpe HonoratoSampaio,
Amilton Paulo Raposo Costa
Publication year - 2021
Publication title -
acta veterinaria brasilica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 8
ISSN - 1981-5484
DOI - 10.21708/avb.2021.15.3.9629
Subject(s) - corpus luteum , theca , antral follicle , medicine , endocrinology , angiotensin ii , ovary , follicular phase , ovulation , ovarian follicle , luteal phase , renin–angiotensin system , biology , andrology , hormone , blood pressure
There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.