Open AccessZEB1 is Negatively Correlated with E-Cadherin in Prostatic Anomaly Tissue
Author(s) -
Sari Eka Pratiwi,
Sri Nuryani Wahyuningrum,
Rachmagreta Perdana Putri,
Danarto Danarto,
Didik Setyo Heriyanto,
Nur Arfian,
Sofia Mubarika Haryana,
Indwiani Astuti
Publication year - 2022
Publication title -
molecular and cellular biomedical sciences
Language(s) - English
Resource type - Journals
eISSN - 2527-4384
pISSN - 2527-3442
DOI - 10.21705/mcbs.v6i1.220
Subject(s) - cadherin , hyperplasia , prostate , prostate cancer , messenger rna , malignancy , epithelial–mesenchymal transition , cancer research , downregulation and upregulation , correlation , metastasis , intraepithelial neoplasia , medicine , chemistry , biology , cancer , pathology , cell , gene , mathematics , biochemistry , geometry
Background: Prostatic anomalies are common in tumor or infection condition. The enlargement of prostate gland affects the epithelial cell polarity that involves epithelial-mesenchymal transition (EMT). Transition into mesenchymal is mediated by transcription factor ZEB1 and E-cadherin protein. Upregulation of ZEB1 and loss of E-Cadherin expression were associated to proliferation and metastasis of malignancy cells. This study aims to describe the correlation of ZEB1 and E-cadherin expression in prostatic anomaly.Materials and method: Samples were Formalin Fixed Paraffin Embedded (FFPE) block consist of 8 block Benign Prostatic Hyperplasia (BPH), 6 blocks High Grade Prostatic Intraepithelial Neoplasia (HGPIN) and 6 blocks Prostate Carcinoma (PCA). The blocks then sliced into 5 sections to be prepared for RNA extraction procedures. ZEB1 and E-Cadherin expression was analyzed by semi-quantitative procedures using PCR and electrophoresis. Correlation between ZEB1 and E-Cadherin espression was analyzed using Spearman’s rank correlation.Results: Relative expression of ZEB1 and E-cadherin mRNA in each group of prostatic anomaly were not significantly different (p>0.05). ZEB1 and E-Cadherin mRNA expression showed a significant and moderate level of negative correlation (p<0.05; 0.40 < r < 0.59). Increasing of ZEB1 mRNA expression will be followed by decreasing of E-Cadherin mRNA expression.Conclusion: ZEB1 negatively correlates with E-cadherin due to EMT process in prostatic anomaly. High expression of ZEB1 induced down-regulation of E-cadherin and vise versa. Various studies can be developed, especially the development of targeted therapy against ZEB1 to suppress the EMT process by increasing the expression of E-cadherin.Keywords: epithelial-mesenchymal transition (EMT), ZEB1, E-Cadherin, BPH, HGPIN, PCA