Open AccessDetection of 2,4-Dinitrophenol and Bio-Imaging Application with Agaric-Based Nitrogen-Doped Carbon Quantum Dots (N-CQDs)
Author(s) -
Jiao Zhao,
Xiting Long,
Zhihua Li,
Yujiao Tu
Publication year - 2021
Publication title -
journal of the brazilian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.337
H-Index - 70
eISSN - 1678-4790
pISSN - 0103-5053
DOI - 10.21577/0103-5053.20210110
Subject(s) - quenching (fluorescence) , carbon fibers , fluorescence , detection limit , chemistry , nitrogen , ionic strength , analytical chemistry (journal) , nuclear chemistry , materials science , inorganic chemistry , composite number , environmental chemistry , organic chemistry , chromatography , aqueous solution , composite material , physics , quantum mechanics
With agaric as carbon source and urea as nitrogen source, this paper has successfully synthesized nitrogen-doped carbon quantum dots (N-CQDs) by simple one-step hydrothermal method. A series of analysis have been used to make characterization of the physical, chemical structures and optical performance of N-CQDs. The fluorescence stability was studied by examining the ionic strength, pH value and storage time and other conditions. The cytotoxicity and bacterial toxicity of N-CQDs were applied to biological imaging. Considering the quenching effect of 2,4-dinitrophenol (2,4‑DNP) on the fluorescence generated by N-CQDs, N-CQDs have been used as fluorescence probe in detection of 2,4-DNP where N-CQDs showed high sensitivity, selectivity and strong disturbance resistance to 2,4-DNP based on the quenching mechanism of transfer of resonance energy. It can also be concluded that agaric-based N-CQDs can be used in bio-imaging due to a good inhibitory effect on but low toxicity to bacteria. Under the optimal experimental conditions, the probe has presented a good coefficient of determination (R2 = 0.9969) and a low limit of detection (15.78 nM). This method has been successfully applied to determine 2,4-DNP in environmental samples and provided a new idea for developing green and natural composite carbon materials.